The University of San Agustin first offered the BS Medical Technology Course (now known as BS Medical Laboratory Science) in the Academic Year 1962-1963. It is a 4-year course with the first 3 years as preparatory to the internship training given in the 4th year. The University has its own tertiary training laboratory for this purpose that is duly accredited by the Department of Health and the Commission on Higher Education. Affiliations have been established with several training hospitals so that interns can have the feel of the actual work of Medical Technologists.
 
The MLS program of the University exceeds the minimum requirement set by related regulatory government agencies. It strives to constantly update itself to maintain the academic excellence that has brought the University to the local, national and international scene. The curricular offerings from the basic to core and professional courses are regularly monitored to keep the program attuned to the needs of the industries and emerging markets. MLS graduates of the University of San Agustin are highly competitive and efficient imbued with Augustinian values making them extremely sought-after.
 
This program made a name for the University for more than 50 years and is still doing so. Despite the numerous obstacles over the years, the program is way moving steadily in its course from its early days to the turn of millennium to the 2nd decade of the 21st century producing quality, excellent Medical Technologist (imbued with the Augustinian Graduate Attributes: A transformative community builder oriented towards GOD; A restless critical and creative thinker; A social communicator of the truth), not only in the local and national scene but even in the international forefront.